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human recombinant magl  (Cayman Chemical)


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    Cayman Chemical human recombinant magl
    Human Recombinant Magl, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+magl/human+recombinant+magl/pm40554889-567-0-10
    Average 90 stars, based on 1 article reviews
    human recombinant magl - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: Identification of a Possible Endocannabinoid-Mediated Mechanism of Action of Cetylated Fatty Acids.
    Article Snippet: .. Human recombinant MAGL and 4-nitrophenyl acetate (4-NPA) were purchased from Cayman Chemical. hMAGL had a ≥95% purity rate estimated by SDSPAGE and was stored at −80 ◦C in order to preserve its stability, as recommended by the producer. ..

    Article Title: Discovery of reversible monoacylglycerol lipase (MAGL) inhibitors based on ortho-hydroxyanilide scaffold.
    Article Snippet: Monoacylglycerol lipase (MAGL) represents a key enzyme in the regulation of the endocannabinoid system and arachidonic acid signaling, emerging as a promising target for the treatment of inflammation, neurodegeneration, and cancer.. Herein, we report the design, synthesis, and biological evaluation of a novel series of ohydroxyanilide derivatives as reversible MAGL inhibitors.. Starting from a known salicylketoxime scaffold, we introduced strategic modifications to reduce lipophilicity and enhance selectivity and potency.

    Article Title: Discovery of Monoacylglycerol Lipase (MAGL) Inhibitors Based on a Pharmacophore-Guided Virtual Screening Study
    Article Snippet: Compounds VS1 – VS7 were bought from their source databases (ChemBridge, Enamine, Pharmeks and Vitas-M corporation). .. Human recombinant MAGL and 4-nitrophenyl acetate substrate (4-NPA) were bought from Cayman Chemical (Ann Arbor, MI, USA). ..

    Article Title: Design, synthesis and biological evaluation of second-generation benzoylpiperidine derivatives as reversible monoacylglycerol lipase (MAGL) inhibitors.
    Article Snippet: An interesting enzyme of the endocannabinoid system is monoacylglycerol lipase (MAGL).. This enzyme, which metabolizes the endocannabinoid 2-arachidonoylglycerol (2-AG), has attracted great interest due to its involvement in several physiological and pathological processes, such as cancer progression.. Experimental evidences highlighted some drawbacks associated with the use of irreversible MAGL inhibitors in vivo, therefore the research field concerning reversible inhibitors is rapidly growing.

    Article Title: Reversible Monoacylglycerol Lipase Inhibitors: Discovery of a New Class of Benzylpiperidine Derivatives
    Article Snippet: .. Human recombinant MAGL and 4-nitrophenylacetate (4-NPA) substrates were purchased from Cayman Chemical. ..

    Article Title: Identification of a Possible Endocannabinoid-Mediated Mechanism of Action of Cetylated Fatty Acids
    Article Snippet: .. Human recombinant MAGL and 4-nitrophenyl acetate (4-NPA) were purchased from Cayman Chemical. h MAGL had a ≥95% purity rate estimated by SDSPAGE and was stored at −80 °C in order to preserve its stability, as recommended by the producer. ..

    Article Title: Design, synthesis, ADME and biological evaluation of benzylpiperidine and benzylpiperazine derivatives as novel reversible monoacylglycerol lipase (MAGL) inhibitors.
    Article Snippet: .. Human recombinant MAGL and 4-nitrophenylacetate (4-NPA) were commercially available from Cayman Chemical. ..

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..

    Activity Assay:

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..

    Generated:

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..

    Concentration Assay:

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..

    Derivative Assay:

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..

    Software:

    Article Title: Watermelon: setup and validation of an in silico fragment-based approach.
    Article Snippet: .. the compounds identified by the Vs were purchased from aldrich Market select and their inhibitory activity against MaGl was experimentally evaluated as previously reported32. human recombinant MaGl and 4-nitrophenylacetate (4-NPa) were commercially available from cayman chemical. ic50 values were generated in 96-well microtiter plates. the MaGl reaction was performed at room temperature, at a final volume of 200 μl in 10 mM tris buffer, ph 7.2, containing 1 mM eDta and 0.1 mg/ml bovine serum albumin (Bsa). a total of 150 μl of 4-NPa 133.3 μM was added to 10 μl of DMsO containing the suitable amount of compound. the reaction was started by adding 40 μl of MaGl (11 ng/well) so that the assay was linear over 30 min. the final concentration of the tested compounds ranged for from 320 to 0.02 nM. after 30 min from the start of the reaction, the absorbance values were measured by using Victor X3 Microplates Reader (Perkinelmer®) at 405 nm. two reactions were also performed: (1) a reaction containing no compound and (2) a reaction containing neither compound nor MaGl. ic50 values were derived from experimental data using the sigmoidal dose − response fitting of GraphPad Prism software. ..



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    Image Search Results


    Comparison of luminescence produced by ArLuc- 1 in presence (●) or absence (▪) of Monoacylglycerol lipase (MAGL). Data represent mean ± standard deviation (SD).

    Journal: International Journal of Molecular Sciences

    Article Title: A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase

    doi: 10.3390/ijms22116148

    Figure Lengend Snippet: Comparison of luminescence produced by ArLuc- 1 in presence (●) or absence (▪) of Monoacylglycerol lipase (MAGL). Data represent mean ± standard deviation (SD).

    Article Snippet: Monoacylglycerol lipase (human recombinant, 50 μg, h MAGL) was purchased from Cayman Chemical (Ann Arbor, MI, USA).

    Techniques: Comparison, Produced, Standard Deviation